Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYCN

Cell type

Cell type Class
Neural
Cell type
SH-EP
NA
NA

Attributes by original data submitter

Sample

source_name
SHEP MYCN neuroblastoma cell line
input
NONE
treatment
DMSO for 6HR
growth protocol
10% FBS, DMEM
barcode
GGAGCTAC

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using Tn5 enzyme and amplified using 2x NEBNext HiFi master mix.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
40898121
Reads aligned (%)
93.0
Duplicates removed (%)
22.6
Number of peaks
1511 (qval < 1E-05)

hg19

Number of total reads
40898121
Reads aligned (%)
91.9
Duplicates removed (%)
23.3
Number of peaks
1298 (qval < 1E-05)

Base call quality data from DBCLS SRA